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Comparison of 5 Methods and Pathways for Synthesizing siRNA Molecules
At present, researchers use the method of introducing siRNA molecules into cells for RNAi research. There are currently 5 methods and pathways for synthesizing siRNA molecules:1. Chemical synthesis;2. In vitro transcription;3. "Cocktail" method (RNaseIII enzyme digestion of dsRNA);4. In vivo expression of siRNA mediated by plasmids and viral vectors;5. siRNA expression box mediated siRNA in vivo expression. -
siRNA design
How to design effective siRNA has always been a hot topic in current RNAi research The effectiveness of gene suppression largely depends on the selection of the target gene sequence. The target sequence can be randomly selected or tested on different regions of the target gene to determine which sequence is most effective. The following points are extremely important for the success of siRNA design. -
Principles and Applications of RNA Interference Technology (Part 1)
RNAi was first discovered by Fire et al. in C. elegans. They found that injecting dsRNA into the nematode can inhibit the expression of homologous genes, and confirmed that this inhibition mainly acts after transcription. Therefore, RNAi is also known as post transcriptional gene silencing (PTGS). -
The proprietary terms and related names of RNAi technology
RNAi：（RNA interference）RNA干扰：一些小的双链RNA可以高效、特异的阻断体内特定基因表达，促使mRNA降解，诱使细胞表现出特定基因缺失的表型，称为RNA干扰（RNA interference，RNAi，也译作RNA干预或者干涉）。它也是体内抵御外在感染的一种重要保护机制。 -
Origin of RNAi
The first clue that dsRNA can lead to gene silencing came from the study of Caenorhabditis elegans, a nematode. >In 1995, Dr. Su Guo and Kemphue from Cornell University discovered an unexpected phenomenon while attempting to block the par-1 gene in C. elegans. They originally used antisense RNA technology to specifically block the expression of the above-mentioned genes, while injecting sense RNA into nematodes in control experiments in order to observe an increase in gene expression. But the result obtained was that both equally cut off the expression pathway of the par-1 gene. This is exactly opposite to the traditional explanation of antisense RNA technology. The research team has been unable to provide a reasonable explanation for this accident.
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