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Principles and Applications of RNA Interference Technology (Part 1)
RNAi was first discovered by Fire et al. in C. elegans. They found that injecting dsRNA into the nematode can inhibit the expression of homologous genes, and confirmed that this inhibition mainly acts after transcription. Therefore, RNAi is also known as post transcriptional gene silencing (PTGS). -
The proprietary terms and related names of RNAi technology
RNAi：（RNA interference）RNA干扰：一些小的双链RNA可以高效、特异的阻断体内特定基因表达，促使mRNA降解，诱使细胞表现出特定基因缺失的表型，称为RNA干扰（RNA interference，RNAi，也译作RNA干预或者干涉）。它也是体内抵御外在感染的一种重要保护机制。 -
Origin of RNAi
The first clue that dsRNA can lead to gene silencing came from the study of Caenorhabditis elegans, a nematode. >In 1995, Dr. Su Guo and Kemphue from Cornell University discovered an unexpected phenomenon while attempting to block the par-1 gene in C. elegans. They originally used antisense RNA technology to specifically block the expression of the above-mentioned genes, while injecting sense RNA into nematodes in control experiments in order to observe an increase in gene expression. But the result obtained was that both equally cut off the expression pathway of the par-1 gene. This is exactly opposite to the traditional explanation of antisense RNA technology. The research team has been unable to provide a reasonable explanation for this accident.
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Comparison List of Gene Transfection and Transfection Reagents
The data is extracted from the manufacturer's online publication for reference only. The data is subject to the original manufacturer's publication. -
Comparison of various transfection methods for gene transfection
Comparison of various transfection methods, including DEAE glucan method, calcium phosphate method, cationic liposome method, cationic polymer method, virus mediated method, Biolitic particle delivery method (gene gun particle bombardment method), microinjection method, electroporation method, etc., for gene transfection. -
Transfect commonly used reporter genes
A reporter gene is a gene that encodes a detectable protein or enzyme, and its expression product is easily identifiable. Fusing its coding sequence with gene expression regulatory sequence to form a chimeric gene, or fusing it with other target genes, and expressing it under the control of regulatory sequence, so as to use its expression product to calibrate the expression regulation of target genes and screen for transformants.
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